Construction and characterization of human rotavirus recombinant VP8* subunit parenteral vaccine candidates
نویسندگان
چکیده
منابع مشابه
Safety and immunogenicity of a parenteral P2-VP8-P[8] subunit rotavirus vaccine in toddlers and infants in South Africa: a randomised, double-blind, placebo-controlled trial.
BACKGROUND Efficacy of live oral rotavirus vaccines is reduced in low-income compared with high-income settings. Parenteral non-replicating rotavirus vaccines might offer benefits over oral vaccines. We assessed the safety and immunogenicity of the P2-VP8-P[8] subunit rotavirus vaccine at different doses in South African toddlers and infants. METHODS This double-blind, randomised, placebo-con...
متن کاملFine mapping of sequential neutralization epitopes on the subunit protein VP8 of human rotavirus.
The epitopes of the HRV (human rotavirus), especially those involved in virus neutralization, have not been determined in their entirety, and would have significant implications for HRV vaccine development. In the present study, we report on the epitope mapping and identification of sequential neutralization epitopes, on the Wa strain HRV subunit protein VP8, using synthetic overlapping peptide...
متن کاملSubunit Vaccine Preparation of Bovine Rotavirus and Its Efficacy in Mice
Background: Rotaviruses (RV) are important viral diarrheal agents in calves. Vaccination is an optimum measure to prevent bovine rotaviruses (BRV) infection. However, little research on BRV VP7 vaccine has been done and currently there is no BRV vaccine. Objective: To prepare a subunit vaccine of BRV and investigate its efficacy. Methods: Total RNA was extracted from MA104 cells infected with b...
متن کاملDesign and Construction of Chimeric VP8-S2 Antigen for Bovine Rotavirus and Bovine Coronavirus.
PURPOSE Bovine Rotavirus and Bovine Coronavirus are the most important causes of diarrhea in newborn calves and in some other species such as pigs and sheep. Rotavirus VP8 subunit is the major determinant of the viral infectivity and neutralization. Spike glycoprotein of coronavirus is responsible for induction of neutralizing antibody response. METHODS In the present study, several predictio...
متن کاملSerotype-specific epitope(s) present on the VP8 subunit of rotavirus VP4 protein.
cDNA clones representing the VP8 and VP5 subunits of VP4 of symptomatic human rotavirus strain KU (VP7 serotype 1 and VP4 serotype 1A) or DS-1 (VP7 serotype 2 and VP4 serotype 1B) or asymptomatic human rotavirus strain 1076 (VP7 serotype 2 and VP4 serotype 2) were constructed and inserted into the pGEMEX-1 plasmid and expressed in Escherichia coli. Immunization of guinea pigs with the VP8 or VP...
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ژورنال
عنوان ژورنال: Vaccine
سال: 2012
ISSN: 0264-410X
DOI: 10.1016/j.vaccine.2012.07.078